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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
16/04/2004 |
Data da última atualização: |
16/04/2004 |
Autoria: |
ALVAREZ, E.; CHACON, M. I.; SANCHEZ, J. |
Título: |
DNA polymorphism and virulence variation of Phytophthora population isolated from cassava Manihot esculenta Crantz. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 37, nov., 1998. Suplemento. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Cassava (Manihot esculenta Crantz) is the fourth most important source of dietary carbohydrate in developing countries. A major constraint to cassava production in Latin America is rot, a destructive and widespread disease. To effectively breed for root-rot resistance, researchers must understand the nature of root rotcausing pathogens in general and the virulence patterns of Phytophthora in particular. The overall objective of this research was to study the genetic variation of Phytophthora isolates in terms of virunlence and DNA polymorphism. One hundred twenty two Phytophthora isolates were obtained using a direct palting method and a baiting technique in which fragments of cassava plantlets propagated in vitro, were used as bait. All isolates were characterized by morphology, pathogenicity and PCR-RAPD analysis. Seven reference isolates of different Phytophthora species were also included in the molecular analysis(I). We also identified the isolates by enzyme-linked immunosorbent assay (ELISA) and DNA hybridization, using a Phytophthora specific probe(2). Virulence variation was determined by inoculating through wounding, cassava sprouts that represented 10 varietal differentials. The inocula were 30 selected isolates from different Colombian regions. Most isolates were not clearly differentiated on PDA, V8A and oatmeal agar by colony type or growth. Identification of the isolates by morphology was difficult because not all isolates produce fungal structures.Amplification of the ITS (internal Transcribed Spacer) region of the rDNA was obtained with template DNA from 122 isolates using extracted DNA. The amplified product for the ITS region of all species was 900 bp. Restriction digest with Aiul, Mspl and Hindl of the product amplified for the ITS region showed different restriction paftems, which corresponded to the different species tested. Five radom 10 mer primers (Operon Technologies, Inc.) gave reproducible bands. Polymorphisms with these single primers differentiated the isolates. Colombian Phytophthora populations are highly diverse and our isolates grouped into 12 clusters. All the isolates were detected by the DNA hybridization probe, a more sensitive method than the ELISA test for identifying the pathogens. The sysptoms caused by individuals were very similar, although sysmptom severity differed among isolates. Virulence was observed within the Colombian group of isolates, but no correlation between virulence variation and DNA polymorphism was observed. MenosAbstract: Cassava (Manihot esculenta Crantz) is the fourth most important source of dietary carbohydrate in developing countries. A major constraint to cassava production in Latin America is rot, a destructive and widespread disease. To effectively breed for root-rot resistance, researchers must understand the nature of root rotcausing pathogens in general and the virulence patterns of Phytophthora in particular. The overall objective of this research was to study the genetic variation of Phytophthora isolates in terms of virunlence and DNA polymorphism. One hundred twenty two Phytophthora isolates were obtained using a direct palting method and a baiting technique in which fragments of cassava plantlets propagated in vitro, were used as bait. All isolates were characterized by morphology, pathogenicity and PCR-RAPD analysis. Seven reference isolates of different Phytophthora species were also included in the molecular analysis(I). We also identified the isolates by enzyme-linked immunosorbent assay (ELISA) and DNA hybridization, using a Phytophthora specific probe(2). Virulence variation was determined by inoculating through wounding, cassava sprouts that represented 10 varietal differentials. The inocula were 30 selected isolates from different Colombian regions. Most isolates were not clearly differentiated on PDA, V8A and oatmeal agar by colony type or growth. Identification of the isolates by morphology was difficult because not all isolates produce fungal structures.Am... Mostrar Tudo |
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LEADER 03001naa a2200145 a 4500 001 1651914 005 2004-04-16 008 1998 bl uuuu u00u1 u #d 100 1 $aALVAREZ, E. 245 $aDNA polymorphism and virulence variation of Phytophthora population isolated from cassava Manihot esculenta Crantz. 260 $c1998 520 $aAbstract: Cassava (Manihot esculenta Crantz) is the fourth most important source of dietary carbohydrate in developing countries. A major constraint to cassava production in Latin America is rot, a destructive and widespread disease. To effectively breed for root-rot resistance, researchers must understand the nature of root rotcausing pathogens in general and the virulence patterns of Phytophthora in particular. The overall objective of this research was to study the genetic variation of Phytophthora isolates in terms of virunlence and DNA polymorphism. One hundred twenty two Phytophthora isolates were obtained using a direct palting method and a baiting technique in which fragments of cassava plantlets propagated in vitro, were used as bait. All isolates were characterized by morphology, pathogenicity and PCR-RAPD analysis. Seven reference isolates of different Phytophthora species were also included in the molecular analysis(I). We also identified the isolates by enzyme-linked immunosorbent assay (ELISA) and DNA hybridization, using a Phytophthora specific probe(2). Virulence variation was determined by inoculating through wounding, cassava sprouts that represented 10 varietal differentials. The inocula were 30 selected isolates from different Colombian regions. Most isolates were not clearly differentiated on PDA, V8A and oatmeal agar by colony type or growth. Identification of the isolates by morphology was difficult because not all isolates produce fungal structures.Amplification of the ITS (internal Transcribed Spacer) region of the rDNA was obtained with template DNA from 122 isolates using extracted DNA. The amplified product for the ITS region of all species was 900 bp. Restriction digest with Aiul, Mspl and Hindl of the product amplified for the ITS region showed different restriction paftems, which corresponded to the different species tested. Five radom 10 mer primers (Operon Technologies, Inc.) gave reproducible bands. Polymorphisms with these single primers differentiated the isolates. Colombian Phytophthora populations are highly diverse and our isolates grouped into 12 clusters. All the isolates were detected by the DNA hybridization probe, a more sensitive method than the ELISA test for identifying the pathogens. The sysptoms caused by individuals were very similar, although sysmptom severity differed among isolates. Virulence was observed within the Colombian group of isolates, but no correlation between virulence variation and DNA polymorphism was observed. 700 1 $aCHACON, M. I. 700 1 $aSANCHEZ, J. 773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p. 37, nov., 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 23 | |
3. | | FRAIRE-CORDERO, S.; PÉREZ RODRÍGUEZ, P.; PÉREZ-HERNÁNDEZ, P.; CORTEZ-ROMERO, C.; GALLEGOS-SÁNCHEZ, J. Reproductive response of Pelibuey sheep to the application of recombinant bovine somatotropin and a metabolic restorative preparation. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 12, p. 1392-1398, Dez. 2018. Título em português: Resposta reprodutiva de ovelhas Pelibuey à aplicação de somatotropina bovina recombinante e de um reconstituinte metabólico.Biblioteca(s): Embrapa Unidades Centrais. |
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7. | | VALICENTE, F. H.; PAIVA, E.; VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F. de; SANCHEZ, J.; BRAVO, A. Characterization by PCR analysis of cryl genes from Bacillus Thuringiensis strains efficient against fall armyworm, Spodoptera frugiperda. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 5., 1997, Singapore. Book of abstracts... Singapore: International Society for Plant Molecular Biology, 1997.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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9. | | MIRABALLESA, C.; SANCHEZ, J.; BARROS, A. T. M. de; HITATEGUYB, S.; MARENOB, P.; SAPORITIA, T.; RIET-CORREA, F. Influence of selective treatment of bulls on the infestation of Haematobia irritans on untreated cows. Veterinary Parasitology, v. 260, p. 58-62, 2018Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Corte. |
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10. | | MORAES, D. P.; LOZANO-SÁNCHEZ, J.; MACHADO, M. L.; VIZZOTTO, M.; LAZZARETTI, M.; LEYVA-JIMENEZ, F. J. J.; SILVEIRA, T. da; RIES, E. F.; BARCIA, M. T. Characterization of a new blackberry cultivar BRS Xingu: chemical composition, phenolic compounds, and antioxidant capacity in vitro and in vivo. Food Chemistry, v. 322, 126783, Aug. 2020. 9 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Clima Temperado. |
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11. | | SILVA, I. H. S. da; GOMÉZ, I.; SÁNCHEZ, J.; CASTRO, D. L. M. de; VALICENTE, F. H.; SOBERÓN, M.; POLANCZYK, R. A.; BRAVO, A. Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin. Plos One, San Francisco, v. 13, n. 12, e0207789, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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12. | | SILVA, I. H. S.; GOMEZ, I.; SANCHEZ, J.; CASTRO, D. L. M.; VALICENTE, F. H.; SOBERON, M.; POLANCZYK, R. A.; BRAVO, A. Identification of midgut membrane proteins from different instars of Helicoverpa armigera that bind to Cry1Ac toxin. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., 2018, Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018. p. 1348.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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13. | | VAZQUEZ-GONZALEZ, Y.; PRIETO, C.; FILIZOGLU, M. F.; RAGAZZO-SANCHEZ, J. A.; CALDERON-SANTOYO, M.; FURTADO, R. F.; CHENG, H. N.; BISWAS, A.; AGARON, J. M. Electrosprayed cashew gum microparticles for the encapsulation of highly sensitive bioactive materials. Carbohydrate Polymers, v. 264, art. no. 118060, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agroindústria Tropical. |
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14. | | GÓMEZ, I.; SÁNCHEZ, J.; LIMA, C.; MARTINS, E.; ROSALES-JUÁREZ, A.; AGUILAR-MEDEL, S.; ABAD, A.; DONG, H.; PONTES, R. G. M. S. de; PEÑA, G.; ZHANG, J.; NELSON, M.; WU, G.; BRAVO, A.; SOBERÓN, M. Enhancement of Bacillus thuringiensis Cry1Ab and Cry1Fa toxicity to Spodoptera frugiperda by domain III mutations indicates there are two limiting steps in toxicity as defined by receptor binding and protein stability Applied and Environmental Microbiology, v. 84, n. 20, article e01393-18, 2018. Na publicação: Rose Monnerat.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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15. | | RODRIGUEZ, C. A.; CHAGAS, E. A.; ALMEIDA, L. F. P. de; DELGADO, J. P. M.; TORRES, D. del C.; PANDURO, M. P.; SILVA, V. E. C. da; SANCHEZ, J. S. C. Genotype and Grafting Techniques Effects on Survival and Growth of Camu Camu Plants Journal of Agricultural Science, v. 7, n. 6, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Roraima. |
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16. | | MORAES, D. P.; MACHADO, M. L.; FARIAS, C. A. A.; BARIN, J. S.; ZABOT, G. L.; LOZANO-SÁNCHEZ, J.; FERREIRA, d. f.; VIZZOTTO, M.; LEYVA-JIMENEZ, F. J.; SILVEIRA, T. L. DA; RIES, E. F.; BARCIA, M. T. Effect of microwave hydrodiffusion and gravity on the extractionof phenolic compounds and antioxidant properties of blackberries (Rubusspp.): scale-Up extraction. Food and Bioprocess Technology, v. 13, p. 2200-2216, 2020. Published online: 20 November 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Clima Temperado. |
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17. | | MARTINS, E. S.; RIBEIRO, B. M.; QUEIROZ, P. R.; DUMAS, V. F.; BRAZ, S. V.; AGUIAR, R. W. S.; GOMES, A. C. M. M.; SÁNCHEZ, J.; BRAVO, A.; PONTES, R. G. M. S. de. Primeiro relato de identificação de receptor para toxinas de Bacillus thuringiensis EM Anthonomus grandis. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 085. p. 126Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | MONNERAT, R.; MARTINS, E.; QUEIROZ, P.; ORDÚZ, S.; JARAMILLO, G.; BENINTENDE, G.; COZZI, J.; REAL, M. D.; MARTINEZ-RAMIREZ, A.; RAUSELL, C.; CERÓN, J.; IBARRA, J. E.; RINCON-CASTRO, M. C. del; ESPINOZA, A. M.; MEZA-BASSO, L.; CABRERA, L.; SÁNCHEZ, J.; SOBERON, M.; BRAVO, A. Genetic variability of Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) populations from Latin America is associated with variations in susceptibility to Bacillus thuringiensis cry toxins. Applied and Environmental Microbiology, v. 72, n. 11, p. 7029-7035, 2006.Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - A |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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19. | | BAROIS, I.; VELASQUEZ, E.; LAVELLE, P.; NEGRETE-YANKELEVICH, S; GARCÍA, J. A.; SANTOS, M.; ÁLVAREZ-SÁNCHEZ, J.; CASTILLO-CAMPOS, G.; CRAM, S.; FRAGOSO, C; FRANCO-NAVARRO, F.; MARTÍNEZ-ROMERO, E.; MEZA, E.; MORÓN, M. A.; RODRÍGUEZ, P.; ROJAS, P.; SOSA, V.; TREJO, D.; VARELA, L.; BUENO-VILLEGAS, J.; GÓMEZ, J. A.; SORMANI, C. Soil quality parameters that determine the below ground biodiversity in Los Tuxtlas Veracruz Mexico. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.Biblioteca(s): Embrapa Florestas. |
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20. | | MARTINS, E. S.; MONNERAT, R. G.; QUEIROZ, P. R.; ORDUZ, S.; BENINTENDE, G.; COZZI, J.; REAL, M. D.; MARTINEZ-RAMIREZ, A.; RAUSELL, C.; CERÓN, J.; IBARRA, J. E.; RINCON-CASTRO, M. C. D.; ESPINOZA, A. M.; MEZA-BASSO, L.; CABRERA, L.; SÁNCHEZ, J.; SOBERON, M.; BRAVO, A. A variabilidade genética em populações de Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) da América Latina está associada a variações de suceptibilidade a toxinas Cry de Bacillus thuringiensis. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 122.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 23 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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